cfps kit rts 100 e. coli hy Search Results


cfps kit rts 100 e. coli hy kit  (5 PRIME)
90
5 PRIME cfps kit rts 100 e. coli hy kit
Cell-free expression of EmGFP using the <t>CFPS</t> <t>kit.</t> ( A ) Buffer dependence. Activity was determined using the indicated buffers. The reaction volumes were diluted (dilution factor of 1.5) to 15 μl and measured in the fluorometer every 20 s. ( B ) Plasmid dependence. Activity was determined, using the buffer provided with the kit for the indicated plasmids. Anti-tmRNA oligo DNA at a concentration of 5 μM was added in addition to the linearized plasmid to prevent dissociation of the ribosome-EmGFP complex. All experiments were preformed in triplicate. Signal was acquired every 45 s, avoiding sample irradiation between consecutive measurements in order to minimize photobleaching.
Cfps Kit Rts 100 E. Coli Hy Kit, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfps+kit+rts+100+e%2E+coli+hy/perfectpure+rna+cultured+cell+kit/pmc03384345-55-1-19
Average 90 stars, based on 1 article reviews
cfps kit rts 100 e. coli hy kit - by Bioz Stars, 2026-10
90/100 stars
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rapid translation system rts 100 e. coli hy kit  (5 PRIME)
90
5 PRIME rapid translation system rts 100 e. coli hy kit
Cell-free expression of EmGFP using the <t>CFPS</t> <t>kit.</t> ( A ) Buffer dependence. Activity was determined using the indicated buffers. The reaction volumes were diluted (dilution factor of 1.5) to 15 μl and measured in the fluorometer every 20 s. ( B ) Plasmid dependence. Activity was determined, using the buffer provided with the kit for the indicated plasmids. Anti-tmRNA oligo DNA at a concentration of 5 μM was added in addition to the linearized plasmid to prevent dissociation of the ribosome-EmGFP complex. All experiments were preformed in triplicate. Signal was acquired every 45 s, avoiding sample irradiation between consecutive measurements in order to minimize photobleaching.
Rapid Translation System Rts 100 E. Coli Hy Kit, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfps+kit+rts+100+e%2E+coli+hy/rts+100+e++coli+hy+kit/pmc08868817-129-17-37
Average 90 stars, based on 1 article reviews
rapid translation system rts 100 e. coli hy kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

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Cell-free expression of EmGFP using the CFPS kit. ( A ) Buffer dependence. Activity was determined using the indicated buffers. The reaction volumes were diluted (dilution factor of 1.5) to 15 μl and measured in the fluorometer every 20 s. ( B ) Plasmid dependence. Activity was determined, using the buffer provided with the kit for the indicated plasmids. Anti-tmRNA oligo DNA at a concentration of 5 μM was added in addition to the linearized plasmid to prevent dissociation of the ribosome-EmGFP complex. All experiments were preformed in triplicate. Signal was acquired every 45 s, avoiding sample irradiation between consecutive measurements in order to minimize photobleaching.

Journal: Nucleic Acids Research

Article Title: Real-time assay for testing components of protein synthesis

doi: 10.1093/nar/gks232

Figure Lengend Snippet: Cell-free expression of EmGFP using the CFPS kit. ( A ) Buffer dependence. Activity was determined using the indicated buffers. The reaction volumes were diluted (dilution factor of 1.5) to 15 μl and measured in the fluorometer every 20 s. ( B ) Plasmid dependence. Activity was determined, using the buffer provided with the kit for the indicated plasmids. Anti-tmRNA oligo DNA at a concentration of 5 μM was added in addition to the linearized plasmid to prevent dissociation of the ribosome-EmGFP complex. All experiments were preformed in triplicate. Signal was acquired every 45 s, avoiding sample irradiation between consecutive measurements in order to minimize photobleaching.

Article Snippet: The CFPS kit (RTS 100 E. coli HY kit – previously sold by Roche Applied Science, currently sold by 5prime) was utilized to synthesize EmGFP according to the manufacturer’s manual, with some modifications.

Techniques: Expressing, Activity Assay, Plasmid Preparation, Concentration Assay, Irradiation